Guidelines

Questionnaires for individuals and population samples

  • Questionnaire for the characterisation and description of individuals in samples of populations for use in anthropologic studies. Get questionnaire (PDF)
  • Questionnaire for the characterisation and description of human population samples for use in anthropologic studies. Get questionnaire (PDF)

Guidelines for reporting HLA typings

As defined at HLA-NET meeting on March 17th – 18th 2011 in Athens

Typing resolution


Whenever possible, perform allelic or high resolution typing following EFI standard D1.320 a and the document “HARMONISATION OF DEFINITIONS OF HISTOCOMPATIBILITY TYPING TERMS” (see hla.alleles.org and Nunes et al. (2011)).

  1. Allelic resolution is a DNA-based typing result consistent with a single allele as defined in a given version of the WHO HLA Nomenclature Report.
  2. High resolution is defined as a set of alleles that specify and encode the same protein sequence for the peptide binding region of an HLA molecule and that excludes alleles that are not expressed as cell-surface proteins. It identifies HLA alleles at the resolution level of the 2nd field (formerly 4-digit) or more, at least resolving all ambiguities resulting from polymorphisms located within exons 2 and 3 for class I loci, and exon 2 for class II loci.
  3. Intermediate resolution is defined as a DNA-based typing result that includes a subset of alleles sharing the digits in the first field of their allele name and that excludes some alleles sharing this field.
  4. Low resolution is a DNA-based typing result at the level of the first field (formerly 2-digit) in the DNA based nomenclature. If none of the above resolutions can be achieved, DNA-based low resolution typings are accepted.

Data with ambiguities / high or intermediate resolution


In case allelic resolution is not achieved, data with allelic ambiguities are accepted in the following formats (in preferential order):

  1. List of possible genotypes (i.e. pairwise allelic combinations) e.g. B*08:01:01G,B*15:18:01 or B*08:21,B*15:93 or B*08:35,B*15:10:01 (corresponding to 3 possible combinations)
  2. Allelic strings e.g. B*08:01/21/35,B*15:10/18/93 (corresponding to 9 possible combinations)
  3. NMDP codes e.g. B*08:MDY,B*15:DZBP (corresponding to 9 possible combinations)

How to cite these questionnaires and guidelines

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